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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ZYMO RESEARCH/Direct-zol-96 MagBead RNA/4 x 96 preps/R2102
产品编号:R2102
市  场 价:¥18140.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$907.00
品      牌: ZYMO RESEARCH
公      司:ZYMO RESEARCH
公司分类:
ZYMO RESEARCH/Direct-zol-96 MagBead RNA/4 x 96 preps/R2102
商品介绍
Isolate total RNA and small/miRNA from TRIzol without phase separation
Highlights

  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.
Description

The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well),magnetic bead-based isolation of high-quality RNA directly from samples in TRIReagent® and similar.The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.Learn More


CompatibilityTRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
EquipmentMicrocentrifuge, vortex, magstand
Sample InactivationTRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample SourceAny sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size RangeTotal RNA ≥ 17 nt
Yield10 µg RNA (binding capacity), ≥ 30 µl (elution volume)

Q1: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q2: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q3: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q4: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q5: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q10: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.




“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME!It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”

-A. Newhart (The Wistar Institute)

“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”

-Mohan K. (University of Illinois, Chicago)

“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”

-Arjan V. (Indiana University)
Read More

Cat #NameSizePrice
D4100-2-3MagBinding Beads3 ml$66.00
D4100-2-12MagBinding Beads12 ml$125.00
W1001-30DNase/RNase-Free Water30 ml$22.00
R1060-2-100RNA Prep Buffer100 ml$122.00
R1060-2-25RNA Prep Buffer25 ml$40.00
R2100-1-20Direct-zol Binding Buffer Concentrate20 ml$84.00
R2100-2-200Direct-zol MagBead PreWash200 ml$174.00
R2130-1-120MagBead DNA/RNA Wash 1120 ml$198.00
R2130-1-30MagBead DNA/RNA Wash 130 ml$63.00
R2130-2-20MagBead DNA/RNA Wash 220 ml$54.00
R2130-2-80MagBead DNA/RNA Wash 280 ml$171.00
C2002Collection Plate2 Plates$22.00
C2003Elution Plate2 Plates$19.00
C2007-896-Well Plate Cover Foil8 Foils$18.00
R2050-1-200TRI Reagent200 ml$219.00
E1010-1-4DNA Digestion Buffer4 mL$15.00
E1010DNase I Set250 U$56.00
品牌介绍

Zymo Research的核心信念基于创新,质量和客户服务。这三个准则根植于他们的公司文化中。他们相信自己的产品是业内最可靠,最严格的最高质量标准,这已通过ISO 9001认证证明。他们还因表观遗传学产品质量的领导力而获得Frost&Sullivan的最佳实践奖。

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