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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ZYMO RESEARCH/Zymoprep-96 Yeast Plasmid Miniprep/4 x 96 Preps/D2006
产品编号:D2006
市  场 价:¥11900.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>其他试剂>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$595.00
品      牌: ZYMO RESEARCH
公      司:ZYMO RESEARCH
公司分类:
ZYMO RESEARCH/Zymoprep-96 Yeast Plasmid Miniprep/4 x 96 Preps/D2006
商品介绍
Simple solution for yeast plasmid DNA isolation using Zymolyase
Highlights

  • Simple: Quickly and easily rescue plasmid from yeast.
  • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
  • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description

The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.


Applicable ForPlasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume≥ 10 µl per well
EquipmentCentrifuge with microplate carriers
Processing Volume≤ 1.5 ml of Culture
Sample SourceCell Culture (Colonies/Patches or Liquid Culture)
Size RangeUp to 25 kb.
YieldTypically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?

Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

Q2: What is the typical plasmid yield?

Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

Q3: Can this kit be used to isolate linear plasmid DNA?

Yes

Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?

We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

Q5: What yeast strains are these kits compatible with?

Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya KloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis



Cat #NameSizePrice
D2004-1-45Solution 1 Digestion Buffer45 ml$88.00
D2004-1-90Solution 1 Digestion Buffer90 ml$146.00
D2004-2-45Solution 2 Lysis Buffer45 ml$88.00
D2004-2-90Solution 2 Lysis Buffer90 ml$146.00
D2004-3-180Solution 3 Neutralizing Buffer180 ml$146.00
D2004-3-90Solution 3 Neutralizing Buffer90 ml$88.00
D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00
D4003-2-48DNA Wash Buffer (Concentrate)48 ml$60.00
C1001-50Collection Tubes50 Pack$15.00
C2002Collection Plate2 Plates$22.00
C2003Elution Plate2 Plates$19.00
C2004Zymo-Spin I-96 Plate2 Plates$142.00
P1001-296-Well Block2 Blocks$18.00
C2007-896-Well Plate Cover Foil8 Foils$18.00
C2011-2Air Permeable Sealing Cover2 Pack$12.00
C2011-4Air Permeable Sealing Cover4 Pack$24.00
C2011-8Air Permeable Sealing Cover8 Pack$42.00
品牌介绍

Zymo Research的核心信念基于创新,质量和客户服务。这三个准则根植于他们的公司文化中。他们相信自己的产品是业内最可靠,最严格的最高质量标准,这已通过ISO 9001认证证明。他们还因表观遗传学产品质量的领导力而获得Frost&Sullivan的最佳实践奖。

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