Applicable For | Next-Generation Sequencing, qPCR, microarray |
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Device Specs | 12 x 80 mm screwcap vial pre-filled with DNA/RNA Shield (1 mL or 2 mL) |
Device Storage | Ambient temperature => 2 years |
Sample Collection | 10% (v/v or w/v) |
Sample Source | Nose, mouth, throat, biological fluids, environmental samples |
Sample Stability | RNA: Ambient temperature (4°C-25°C) > 1 monthDNA: Ambient temperature (4°C-25°C) > 2 years DNA & RNA: Frozen (<-20°C): Indefinitely |
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
RNA > 1 month DNA > 2 years
DNA & RNA (<-20°c):>-20°c):>
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
No, tissues do not need to be homogenized.
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
Zymo Research的核心信念基于创新,质量和客户服务。这三个准则根植于他们的公司文化中。他们相信自己的产品是业内最可靠,最严格的最高质量标准,这已通过ISO 9001认证证明。他们还因表观遗传学产品质量的领导力而获得Frost&Sullivan的最佳实践奖。